The Cheer-off is a competition that many species of animals joined, which every contestant is to cheer.
- Many unnamed clams
- The winners were the Clamettes, but the most spirited cheering squad was the Freshwater High cheerleaders ("We've Got Fish Spirit").
- Coach Salmons is a leader of the Cheer-Off, and possibly is founder ("We've Got Fish Spirit").
- Coach Salmons sings the anthem ("We've Got Fish Spirit")
- "We've Got Fish Spirit" (First appearance)